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Studies in dopamine-depleted rats indicate that the external globus pallidus (GPe) contains two main types of GABAergic projection cell; so-called "prototypic" and "arkypallidal" neurons. Here, we used correlative anatomical and electrophysiological approaches in rats to determine whether and how this dichotomous organization applies to the dopamine-intact GPe. Prototypic neurons coexpressed the transcription factors Nkx2-1 and Lhx6, comprised approximately two-thirds of all GPe neurons, and were the major GPe cell type innervating the subthalamic nucleus (STN). In contrast, arkypallidal neurons expressed the transcription factor FoxP2, constituted just over one-fourth of GPe neurons, and innervated the striatum but not STN. In anesthetized dopamine-intact rats, molecularly identified prototypic neurons fired at relatively high rates and with high regularity, regardless of brain state (slow-wave activity or spontaneous activation). On average, arkypallidal neurons fired at lower rates and regularities than prototypic neurons, and the two cell types could be further distinguished by the temporal coupling of their firing to ongoing cortical oscillations. Complementing the activity differences observed in vivo, the autonomous firing of identified arkypallidal neurons in vitro was slower and more variable than that of prototypic neurons, which tallied with arkypallidal neurons displaying lower amplitudes of a "persistent" sodium current important for such pacemaking. Arkypallidal neurons also exhibited weaker driven and rebound firing compared with prototypic neurons. In conclusion, our data support the concept that a dichotomous functional organization, as actioned by arkypallidal and prototypic neurons with specialized molecular, structural, and physiological properties, is fundamental to the operations of the dopamine-intact GPe.

Original publication

DOI

10.1523/JNEUROSCI.4662-14.2015

Type

Journal article

Journal

J Neurosci

Publication Date

29/04/2015

Volume

35

Pages

6667 - 6688

Keywords

anatomy, arkypallidal, basal ganglia, electrophysiology, globus pallidus, transcription factor, Action Potentials, Adrenergic Agents, Animals, Animals, Newborn, Dopamine, ELAV Proteins, ELAV-Like Protein 3, Female, Forkhead Transcription Factors, Globus Pallidus, Green Fluorescent Proteins, In Vitro Techniques, Neural Pathways, Neurons, Nuclear Proteins, Oxidopamine, Parvalbumins, Rats, Statistics, Nonparametric, Subthalamic Nucleus, Thyroid Nuclear Factor 1, Transcription Factors