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Clonal microbial cells do not behave in an identical manner and form subpopulations during cultivation. Besides varying micro-environmental conditions, cell inherent features like cell cycle dependent localization and concentration of regulatory proteins as well as epigenetic properties are well accepted mechanisms creating cell heterogeneity. Another suspected reason is molecular noise on the transcriptional and translational level. A promising tool to unravel reasons for cell heterogeneity is the combination of cell sorting and subpopulation proteomics. This review summarizes recent developments in prokaryotic single-cell analytics and provides a workflow for selection of single cells, low cell number mass spectrometry, and proteomics evaluation. This approach is useful for understanding the dependency of individual cell decisions on inherent protein profiles.

Original publication

DOI

10.1016/j.copbio.2012.10.017

Type

Journal article

Journal

Curr opin biotechnol

Publication Date

02/2013

Volume

24

Pages

79 - 87

Keywords

Clone Cells, Mass Spectrometry, Prokaryotic Cells, Proteome, Proteomics, Single-Cell Analysis